ANTI-SCARRING GENE THERAPY
Publications
Lactoferrin derived anti-scarring peptide
​
Håkansson J, Mahlapuu M, Ekström L, Olmarker K, Wiig M., Effect of lactoferrin peptide (PXL01) on rabbit digit mobility after flexor tendon repair. Hand Surg Am. 2012 (12): 2519-25.
​https://journals.sagepub.com/doi/10.1177/1753193411410823
​
Nilsson E, Björn C, Sjöstrand V, Lindgren K, Münnich M, Mattsby-Baltzer I, Ivarsson ML, Olmarker K, Mahlapuu M., A novel polypeptide derived from human lactoferrin in sodium hyaluronate prevents postsurgical adhesion formation in the rat. Ann Surg. 2009, 250(6):1021-8.
​https://www.ncbi.nlm.nih.gov/pubmed/19953722
​
Wiig ME, Dahlin LB, Fridén J, Hagberg L, Larsen SE, Wiklund K, Mahlapuu M., PXL01 in sodium hyaluronate for improvement of hand recovery after flexor tendon repair surgery: randomized controlled trial. PLoS One. 2014 23;9(10):e110735. doi: 10.1371/journal.pone.0110735. eCollection 2014.
​https://lucris.lub.lu.se/ws/files/3843122/7525885
​
​
Scarring and adhesions
​
Dermal Scarring
​
Finnerty CC, Jeschke MG, Branski LK, Barret JP, Dziewulski P, Herndon DN., Hypertrophic scarring: the greatest unmet challenge after burn injury. Lancet. 2016 ;388(10052):1427-1436. Review.
https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(16)31406-4/fulltext
​
Lee HJ, Jang YJ., Recent Understandings of Biology, Prophylaxis and Treatment Strategies for Hypertrophic Scars and Keloids. Int J Mol Sci. 2018 9(3). pii: E711. Review
​https://www.researchgate.net/publication/323529902_Recent_Understandings_of_Biology_Prophylaxis
​
Li-Tsang CW, Lau JC, Chan CC., Prevalence of hypertrophic scar formation and its characteristics among the Chinese population. Burns. 2005,31(5):610-6
​https://www.ncbi.nlm.nih.gov/pubmed/15993306
​
Marshall CD, Hu MS, Leavitt T, Barnes LA, Lorenz HP, Longaker MT., Cutaneous Scarring: Basic Science, Current Treatments, and Future Directions., Adv Wound Care (New Rochelle). 2018 7(2):29-45.
​https://www.liebertpub.com/doi/pdf/10.1089/wound.2018.0787
.
​
Post operative adhesions
​
Awonuga AO, Fletcher NM, Saed GM, Diamond MP., Postoperative adhesion development following cesarean and open intra-abdominal gynecological operations: a review. Reprod Sci. 2011, (12):1166-85. Review
​https://reference.medscape.com/medline/abstract/21775773
Baber Z, Erde MA., Failed back surgery syndrome: current perspectives. J Pain Research, 2016; 9 : 979-987
​
Lue JR , Boyd LS, Diamond MP,. Pelvic Adhesive Disease: Current Approaches to Management, J Womens Health Issues Care 2014; 44(3):405-20. Review.
​
Moris D, Chakedis J, Rahnemai-Azar AA, Wilson A, Hennessy MM, Athanasiou A,. Postoperative Abdominal Adhesions: Clinical Significance and Advances in Prevention and Management. Journal of Gastrointestinal Surgery, 2017, 10: 1713–1722
https://link.springer.com/article/10.1007%2Fs11605-017-3488-9
Okabayashi K, Ashrafian H, Zacharakis E, Hasegawa H, Kitagawa Y, Athanasiou T, Darzi A., Adhesions after abdominal surgery: a systematic review of the incidence, distribution and severity. Surg Today, 2014, 44(3):405-20
​Gene therapy
Gorell E, Nguyen N, Lane A, Siprashvili Z., Gene therapy for skin diseases. Cold Spring Harb Perspect Med. 2014 Apr 1;4(4):a015149. doi: 10.1101/cshperspect.a015149. Review.
​https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3968787/pdf/cshperspectmed-SKN-a015149.pdf
​
Kulmala HK, Van Lieshout LP, Thompson B, and Wootton S K. 2020., Manufacture of an AAV6 Triple Mutant Ensereptide-Fc Vector and in Vivo Expression of the Peptide in Mice Following Intramuscular Administration. Cellastra report.
Naso MF, Tomkowicz B, Perry WL 3rd, Strohl WR., Adeno-Associated Virus (AAV) as a Vector for Gene Therapy. BioDrugs. 2017, (4):317-334. doi: 10.1007/s40259-017-0234-5. Review.
​https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548848/pdf/40259_2017_Article_234.pdf
​
​Rghei AD, Brenna AY, Stevens BAY, Thomas SP, Yates JGE, McLoud BM, Karimi K, Susta L, Bridle BW, Wootton SK., Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models. J VIS EXP.
2021 Jun 30:(172).doi: 10.3791/62727.
​
​Rghei AD, van Lieshout LP, Cao W, He S, Tierney K, Lopes JA, Zielinska N, Baracuhy EM, Campbell ESB, Minott JA, Guilleman MM, Hasson PC , Thompson B, Karimi K, Bridle BY, Susta L, Qiu X, Banadyga L, Wootton SK., Adeno-associated virus mediated expression of monoclonal antibody MR191 protects mice against Marburg virus and provides long-term expression in sheep. Gene Ther. 2025 Jan;32(1):50-59. doi: 10.1038/s41434-022-00361-2. Epub 2022 Sep 1.
https://www.nature.com/articles/s41434-022-00361-2
​
Rghei, AD, van Lieshout LP, McLeod BM, Yanlong Pei, Jordyn A Lopes, Nicole Zielinska, Enzo M Baracuhy, et al. 2021., Safety and Tolerability of the Adeno-Associated Virus Vector, AAV6.2FF, Expressing a Monoclonal Antibody in Murine and Ovine Animal Models. Biomedicines.
https://doi.org/10.3390/biomedicines9091186.
​
​van Lieshout LP, Domm JM, Rindler TN, Frost KL, Sorensen DL, Medina SJ, Booth SA, Bridges JP, Wootton SK. A Novel Triple-Mutant AAV6 Capsid Induces Rapid and Potent Transgene Expression in the Muscle and Respiratory Tract of Mice. Mol Ther Methods Clin Dev.
2018 Apr 14:9:323-329.doi: 10.1016/j.omtm.2018.04.005. eCollection 2018 Jun 15.
https://pmc.ncbi.nlm.nih.gov/articles/PMC6054702/
​
van Lieshout LP, Rghei AD, Cao W, He S, Soule G, Zhu W, Thomas SP, Sorensen D, Frost K, Tierney K, Thompson B, Booth S, Safronetz D, Kulkarni RR, Bridle BW, Qiu X, Banadyga L, Wootton SK., AAV-monoclonal antibody expression protects mice from Ebola virus without impeding the endogenous antibody response to heterologous challenge. Mol Ther Methods Clin Dev. 2022 Aug 12:26:505-518.
doi: 10.1016/j.omtm.2022.08.003. eCollection 2022 Sep8.
https://www.sciencedirect.com/science/article/pii/S2329050122001139
​